br Conflicts of interest br Authors declare no conflicts of
Conflicts of interest
Authors declare no conflicts of interest.
This work was supported principally by the Department of Biotechnology (Bio-CARe scheme, BT/BioCARe/07/10003/2013-2014) to author Swati Biswas.
Appendix A. Supplementary data
Bhadra, D., Bhadra, S., Jain, N., 2005. Pegylated lysine based copolymeric dendritic 298186-80-8 for solubilization and delivery of artemether. J. Pharm. Pharm. Sci. 8, 467–482.
Biswas, S., Dodwadkar, N.S., Piroyan, A., Torchilin, V.P., 2012. Surface conjugation of triphenylphosphonium to target poly(amidoamine) dendrimers to mitochondria. Biomaterials 33, 4773–4782.
D'emanuele, A., Jevprasesphant, R., Penny, J., Attwood, D., 2004. The use of a dendrimer-propranolol prodrug to bypass efflux transporters and enhance oral bioavailability. J. Control. Release 95, 447–453.
Duncan, R., Izzo, L., 2005. Dendrimer biocompatibility and toxicity. Adv. Drug Deliv.
S.V.K. Rompicharla et al.
Jansen, J.F., Meijer, E., 1994. Encapsulation of guest molecules into a dendritic box.
Minko, T., Paranjpe, P.V., Qiu, B., Lalloo, A., Won, R., Stein, S., Sinko, P.J., 2002. Enhancing the anticancer efficacy of camptothecin using biotinylated poly (ethyle-neglycol) conjugates in sensitive and multidrug-resistant human ovarian carcinoma cells. Cancer Chemother. Pharmacol. 50, 143–150.
2016. Biotin/folate-decorated human serum albumin nanoparticles of docetaxel: comparison of chemically conjugated nanostructures and physically loaded nano-particles for targeting of breast cancer. Chem. Biol. Drug Des. 87, 69–82.
and curcumin by transferrin-targeted PEG-PE-based mixed micelles on resistant ovarian cancer in 3-D spheroids and in vivo tumors. Eur. J. Pharm. Biopharm. 88, 539–550.
Taheri, A., Dinarvand, R., Nouri, F.S., Khorramizadeh, M.R., Borougeni, A.T., Mansoori, P., Atyabi, F., 2011. Use of biotin targeted methotrexate–human serum albumin conjugated nanoparticles to enhance methotrexate antitumor efficacy. Int. J. Nanomed. 6, 1863.
Yang, Y., Roy, A., Zhao, Y., Undzys, E., Li, S.-D., 2017. Comparison of tumor penetration of podophyllotoxin-carboxymethylcellulose conjugates with various chemical com-positions in tumor spheroid culture and in vivo solid tumor. Bioconjug. Chem. 28, 1505–1518.
Contents lists available at ScienceDirect
journal homepage: www.elsevier.com/locate/canlet
BIRC5 is a target for molecular imaging and detection of human pancreatic T cancer
Shi-He Liua,b, Yeahwa Honga, Stephen Markowiaka, Robbi Sanchezd, Justin Creedena,b, John Nemunaitisc, Andrea Kalinoskia, James Willeyc, Paul Erhardte, Jason Leef,g,h, Michael van Damf,g,h, F. Charles Brunicardia,b,∗ a Department of Surgery, University of Toledo College of Medicine and Life Sciences, Toledo, OH, 43614, USA
b Department of Cancer Biology, University of Toledo College of Medicine and Life Sciences, Toledo, OH, 43614, USA
c Department of Medicine, University of Toledo College of Medicine and Life Sciences, Toledo, OH, 43614, USA
d Department of Surgery, University of California Los Angeles, Los Angeles, CA, 90095, USA
e Department of Pharmacology-Medicinal/Biological Chemistry, University of Toledo College of Medicine and Life Sciences, Toledo, OH, 43614, USA
f Crump Institute for Molecular Imaging, University of California Los Angeles, Los Angeles, CA, 90095, USA
g Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, CA, 90095, USA
h Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, CA, 90095, USA
Thymidine kinase, microPET/CT imaging
Pancreatic ductal adenocarcinoma (PDAC) is a major cause of cancer mortality with a dismal overall survival rate and an urgent need for detection of minute tumors. Current diagnostic modalities have high sensitivity and specificity for larger tumors, but not for minute PDAC. In this study, we test the feasibility of a precision di-agnostic platform for detecting and localizing minute human PDAC in mice. This platform includes: 1) defining BIRC5 as an early PDAC-upregulated gene and utilizing an enhanced BIRC5 super-promoter to drive expression of dual Gaussia luciferase (GLuc) and sr39 thymidine kinase (sr39TK) reporter genes exponentially and speci-fically in PDAC; 2) utilizing a genetically-engineered AAV2RGD to ensure targeted delivery of GLuc and sr39TK specifically to PDAC; 3) using serologic GLuc and sr39TK microPET/CT imaging to detect and localize minute human PDAC in mice. The study demonstrates feasibility of a precision diagnostic platform using an integrated technology through a multiple-stage amplification strategy of dual reporter genes to enhance the specificity and sensitivity of detection and localization of minute PDAC tumors and currently undetectable disease.